Microbiology: A Laboratory Manual, Global Edition
Microbiology is a dynamic science. It is constantly evolving as more information is added to the continuum of knowledge, and as microbiological techniques are rapidly modified and refined. The eleventh edition of Microbiology: A Laboratory Manual continues to provide a blend of traditional methodologies with more contemporary procedures to meet the pedagogical needs of all students studying microbiology. As in previous editions, this laboratory manual provides a wide variety of critically selected and tested experiments suitable for undergraduate students in allied health programs, as well as elementary and advanced general microbiology courses.
This laboratory manual is designed to guide students in the development of manipulative skills and techniques essential for understanding the biochemical structure and function of a single cell. Its main goal is to encourage students to apply these laboratory skills in the vocational field of applied microbiology and allied health or to further pursue the study of life at the molecular level. In this manual, comprehensive introductory material is given at the beginning of each major area of study, and specific explanations and detailed directions precede each experiment. This approach augments, enhances, and reinforces course lectures, enabling students to comprehend more readily the concepts and purposes of each experiment. This also provides a review aid if the laboratory and lecture sections are not taught concurrently.
The manual should also reduce the time required for explanations at the beginning of each laboratory session and thus make more time available for performing the experiments. Finally, care has been taken to design experimental procedures so that the supplies, equipment, and instrumentation commonly found in undergraduate institutions will suffice for their successful execution.
This manual consists of 72 experiments arranged into 16 parts. The experiments progress from those that are basic and introductory, requiring minimal manipulations, to those that are more complex, requiring more sophisticated skills. The format of each experiment is intended to facilitate presentation of the material by the instructor and to maximize the learning experience. To this end, each experiment is designed with the following components.
This introductory section defines the specific principles and/or techniques to be mastered.
This is an in-depth discussion of the microbiological concept or technique and the specific experimental procedure.
Clinical or medical applications that appear within each experiment help students connect what they are learning in lecture with what they are doing in the lab. For students who intend to have careers as nurses or in other allied health fields, Clinical Applications explain the relevance of each lab technique to their career plans.
At the Bench
This section signals the beginning of the experiment, and includes the materials, notes of caution, and procedural instructions—all of the things students will need to know at the bench, during the course of the experiment.
This comprehensive list helps students and instructors prepare for each laboratory session. Materials appear under one of the following headings:
Cultures These are the selected test organisms that have been chosen to demonstrate effectively the experimental principle or technique under study. The choice is also based on their ease of cultivation and maintenance in stock culture.
A complete listing of the experimental cultures and prepared slides is presented in Appendix 6.
Media These are the specific media and their quantities per designated student group. Appendix 3 lists the composition and method of preparation of all the media used in this manual. Reagents These include biological stains as well as test reagents. The chemical composition and preparation of the reagents are presented in Appendices 4 and 5.
Equipment Listed under this heading are the supplies and instrumentation that are needed during the laboratory session. The suggested equipment was selected to minimize expense while reflecting current laboratory technique.
This section provides explicit instructions, augmented by diagrams, that aid in the execution and interpretation of the experiment. A caution icon has been placed in experiments that may use potentially pathogenic materials. The instructor may wish to perform some of these experiments as demonstrations.
These sheets, located at the end of each experiment, facilitate interpretation of data and subsequent review by the instructor. The Observations and Results portion of the report provides tables for recording observations and results, and helps the students draw conclusions from and interpret their data. The Review Questions aid the instructor in determining the student’s ability to understand the experimental concepts and techniques. Questions that call for more critical thinking are indicated by the brain icon.
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